ELFT HÍRADÓ

[Szeminárium koordinátor <sem-admin AT szfki.hu>]

PhD Preliminary DefenseAli Jaafar SadeqHUN-REN Wigner RCP SZFIConfocal Raman 
micro-spectroscopy of collagen-containing tissues: the impact of optical 
clearingTuesday, 23 January 2024, 10:00, KFKI Campus, Bldg. 1, 2nd floor, 
Conference RoomRaman spectroscopy as one of the optical spectroscopic methods 
provides valuable information about the molecular structure and conformation, 
which gains great attention for diagnosis and monitoring of biological 
objects. All biomedical optics including Raman spectroscopy are suffering 
from strong light scattering or limited in-depth light penetration. These 
issues pose significant hindrance to the practical application of the optical 
techniques in clinical practice. In the 90s of the last century, the optical 
clearing technique based on the immersion of the tissue into optical clearing 
agent was introduced and developed to enhance the detection depth using 
optical techniques. In this thesis work, I studied the impact of optical 
clearing method on collagen-related Raman peaks to control the optical 
properties of biological objects, specifically ex vivo porcine dura mater and 
skin-dermis in-depth using confocal Raman micro-spectroscopy. I determined 
the impact of pure glycerol as an optical clearing agent on the intensity of 
collagen-related Raman peak, as well as on dehydration and dissociation of 
the collagen using confocal Raman micro-spectroscopy at various depths 
ranging from 0 to 250 μm and treatment times [1]. In addition, the 
diffusivity of 50% glycerol and the changes in the water content within the 
collagen of the dura mater were monitored at various depths and treatment 
times [2]. The effect of the optical clearing on the Raman spectra recorded 
with 633- and 785-nm excitations in ex vivo porcine skin-dermis was also 
studied at different depths and treatment times, together with the influence 
of dimethyl sulfoxide as an enhancer additive [3].[1] Ali Jaafar, Maxim E. 
Darvin, Valery V. Tuchin and Miklós Veres; Confocal Raman Micro-Spectroscopy 
for Discrimination of Glycerol Diffusivity in Ex vivo porcine dura mater; 
Life 2022, 12(10), 1534; https://doi.org/10.3390/life12101534; Q2; IF: 
3.2.[2] Ali Jaafar, Roman Holomb, Anton Y. Sdobnov, Zsombor Ocskay, Zoltan 
Jakus, Valery V. Tuchin and Miklos Veres; Ex vivo confocal Raman 
microspectroscopy of porcine dura mater supported by optical clearing; 
J.Biophotonics.2022; 15:e202100332.https://doi.org/10.1002/jbio.202100332; 
Q1; IF:2.8.[3] Ali Jaafar, Malik H. Mahmood, Roman Holom, Laszló Himics, 
Tamas Vaczi, Anton Y. Sdobnov|, Valery V. Tuchin and Miklós Veres; Ex-vivo 
confocal Raman microspectroscopy of porcine skin with 633/785-NM laser 
excitation and optical clearing with glycerol/water/DMSO solution; Journal of 
Innovative Optical Health Sciences Vol. 14, No. 05, 2142003,2021, 
https://doi.org/10.1142/S1793545821420037; Q2; IF: 2.5. Minden 
érdeklődőt szívesen látunk! - Everyone is welcome to attend.Attila 
Nagysem-admin AT szfki.hu