ELFT HÍRADÓ

[<vera.horvath AT eli-alps.hu>]

Előadó: Prof. Dr. Joachim Heberle (Freie Universitat, Berlin)
Cím: The grateful infrared – novel IR techniques to probe the functional
changes of membrane proteins

Időpont: 2018. szeptember 4. 14:30
Helyszín: az ELI-ALPS konferenciaterme, Szeged, Budapesti út 5.

Az előadás rövid kivonata:
The catalytic activity of proteins is a function of structural changes. Very
often these are as minute as protonation changes, hydrogen bonding changes and
amino acid side chain reorientations. To resolve these, a methodology is
afforded that not only provides the molecular sensitivity but allows to trace
the sequence of these hierarchical reactions at the same time. I will showcase
results from time-resolved IR spectroscopy [1,2] which was applied to
channelrhodopsin [3].  Channelrhodopsin represents the first light-activated
ion channel to found the basis of the new and exciting field of optogenetics
[4]. I shall also provide an outlook towards novel experimental approaches
like THz pump / IR probe spectroscopy or near-field IR nanoscopy that are
currently developed in my lab [5]. We believe that some of these approaches
have the potential to provide new science.


REFERENCES
[1] Resler T, Schultz BJ, Lórenz-Fonfría VA, Schlesinger R, Heberle J.,
“Kinetic and Vibrational Isotope Effects of Proton Transfer Reactions in
Channelrhodopsin-2”. Biophys J. 109, 287-297 (2015)
[2] Schultz, B.-J., Mohrmann, H., Lórenz-Fonfría, V.A., Heberle J. “Protein
dynamics observed by tunable mid-IR quantum cascade lasers across the time
range from 10 ns to 1 s.” Spectrochim. Acta A 188, 666-674  (2018)
[3] Lorenz-Fonfria, VA, Heberle, J., “Channelrhodopsin unchained: Structure
and mechanism of a light-gated cation channel”. Biochim Biophys Acta 1837,
626-642 (2014)
[4] Adamantidis et al. Nat. Neurosci. 18, 1202-1212 (2015)
[5] Kottke, T., Lórenz-Fonfría V.A., Heberle, J. “The Grateful Infrared –
Sequential Protein Structural Changes Resolved by IR Difference Spectroscopy.”
J. Phys. Chem. B 121, 335-350 (2017)

Az előadás angol nyelvű. Minden érdeklődőt szívesen látunk.