ELFT HÍRADÓ

[Szeminárium koordinátor <sem-admin AT szfki.hu>]

SZFI SeminarRoman HolombHUN-REN Wigner RCP SZFIIdentification of DNA 
sequences and single point variants detection by Raman spectroscopyTuesday, 
19 March 2024, 10:00, KFKI Campus, Bldg. 1, 2nd floor, Conference RoomRaman 
spectroscopy, based on inelastic scattering of photons can successfully be 
used to identify molecules by their intrinsic characteristic vibrations. This 
method is increasingly important in medical sciences, chemical biology, gene 
engineering, pharmaceutical research and recently in the development of 
personalized medicine. The quantitative Raman analysis of macromolecules is 
limited mainly by two factors: (i) the low spontaneous Raman cross-section 
and (ii) the lack of specificity - the vibrational bands are complex and 
depend strongly on the molecular geometry and intermolecular interactions. 
Recently, in biological and medical fields, Raman spectroscopy was 
successfully combined with tagging of the molecules with specific Raman 
active functional groups.Today's click chemistry allows to use the small 
azide or alkynyl groups as bio-orthogonal tags to prevent the alteration of 
the biological activity and other native biochemical processes. The main role 
of this kind of labeling is to obtain high Raman activity and to provide 
characteristic vibrational band(s) in the so called "silent" spectral region 
free from molecular contributions. Our investigations showed that the 
alkyne-tagged reporter molecules can be used to probe the intermolecular 
interactions in complex macromolecules. Ab initio calculations and 
experimental Raman studies showed that the changes in the frequency of the 
alkyne stretching vibration of the reporter molecule provide comprehensive 
information about particular nucleobase pairing, allowing DNA identification 
and single point variants detection. In this talk the developed method and 
its application for Raman detection of gene mutation and viruses will be 
discussed in detail. Minden érdeklődőt szívesen látunk! - Everyone is 
welcome to attend.Attila Nagysem-admin AT szfki.hu