ELFT HÍRADÓ

[Szeminárium koordinátor <szfi-seminar AT wigner.mta.hu>]

SZFI Seminar
Mihály Judith
(Research Centre for Natural Sciences, host: Kamarás Katalin)
Characterization of extracellular vesicles by IR spectroscopy
Extracellular vesicles (EVs) are one of the most attractive and actual 
research area in biology and nanomedicine with growing interest in clinical 
applications. EVs are lipid bilayer-enclosed nanoparticles released by cells. 
They range from 30 nm to several micrometers in diameter, and ferry 
biological cargos such as proteins, lipids, RNAs and DNAs for local and 
distant intercellular communications. Hence, they can serve as early state 
biomarkers of different diseases and/or therapeutic delivery vehicles 
addressing personalized medicine. As they occur in all body fluids (blood, 
urine, saliva), EVs are envisioned as ‘new-generation’ tools for a 
non-invasive and remote ‘liquid biopsy’. Despite intense academic 
investigation, however, the translation of EV-based diagnostics into clinical 
use is not realized, due to the lack of standardized isolation and 
characterization. Infrared spectroscopy (IR) is a label-free method that can 
detect changes of functional groups in molecules from biological samples, 
providing a biochemical fingerprint. Our research group was among the firsts 
performing exploratory IR study on EVs. We selected IR markers to monitor EVs 
formed and accumulated in vitro during the storage of red blood cells (RBCs). 
These IR markers alter in function of storage time and medium, and might be 
indicative for biochemical and morphological changes occurring in blood 
products. Additional information about biomolecular composition (adenosine 
triphosphate, lactose, glucose, oxidized haemoglobin, e.g.) empowers IR 
spectroscopy to be used complementary to traditional omics approaches on EVs. 
By detailed spectral analysis we identified changes in protein structures and 
the relative lipid content for different EV subpopulations. Completed with 
standardized measurement conditions and data processing procedures, IR 
spectroscopy might offer a simple single assay characterization protocol 
providing information about proteins and lipids and/or other EV components 
simultaneously.Tuesday, 22 March 2022, 10:00
Bldg 1, auditorium, 
https://wigner-hu.zoom.us/j/95979470230?pwd=YjdaM3lUSVdDbVBhQmNmb29EdWlLdz09  
 Meeting ID: 959 7947 0230  Passcode: 335878
Language of the talk: English
All interested are welcome!
Róbert Juhászszfi-seminar AT wigner.mta.hu