ELFT HÍRADÓ

[Szeminárium koordinátor <szfi-seminar AT wigner.mta.hu>]

SZFI Seminar
Váczi Tamás
(Wigner FK SZFI)
Development of a spectrally focussed femtosecond SRS system for microscopy 
and spectroscopy
The basic principles and practical aspects of constructing a system for 
spectrally focussed, femtosecond laser-based simulated Raman scattering (SRS) 
applications are presented. The system is based on a turnkey dual-output 
laser unit for rough tuning, glass inserts for pulse chirp, optomechanics for 
controlling spatial and temporal overlap, and a galvanometric scanning 
two-photon microscope with transmitted balanced simulated Raman gain 
detection. Spectral focussing allows fast, high-precision, highly selective 
SRS imaging and spectroscopy through delay tuning but requires the careful 
matching of the chirp parameters of the two pulses. The optimisation of the 
system including the total group delay distortion, balanced detection, the 
acousto-optic modulator transfer function, polarization rotation correction 
etc. are explained, all aiming at increasing the signal-to-noise ratio. 
Demonstrating examples are shown including polymer SRS spectra, label-free 
tissue imaging, concentration series of alkyne-tagged molecules, and a case 
of resonant SRS enhancement.Tuesday, 9 February 2021, 10:00
video conference, https://letsmeet.wigner.hu/szeminarium
Language of the talk: English
All interested are welcome!
Róbert Juhászszfi-seminar AT wigner.mta.hu