ELFT HÍRADÓ

[Szeminárium koordinátor <szfi-seminar AT wigner.mta.hu>]

WIGNER SZFI Szeminárium

Handheld nonlinear microscope system comprising an Yb-fiber laser for in vivo biomedical imaging (Applied Research Prize 2017 of Wigner RCP SZFI)

Szipőcs Róbert
MTA Wigner FK SZFI

2017. december. 19, kedd, 10.00, KFKI Telephely, I. épület 1. emeleti Tanácsterem

A novel, Yb-fiber laser based, handheld 2PEF/SHG microscope imaging system is introduced. It is suitable for in vivo imaging of murine skin at an average power level as low as 5 mW at 200 kHz sampling rate. Amplified and compressed laser pulses having a spectral bandwidth of 8 to 12 nm at around 1030 nm excite the biological samples at a ~1.89 MHz repetition rate, which explains how the high quality two-photon excitation fluorescence (2PEF) and second harmonic generation (SHG) images are obtained at the average power level of a laser pointer. The scanning, imaging and detection head, which comprises a conventional microscope objective for beam focusing, has a physical length of ~180 mm owing to the custom designed imaging telescope system between the laser scanner mirrors and the entrance aperture of the microscope objective. Operation of the all-fiber, all-normal dispersion Yb-fiber ring laser oscillator is electronically controlled by a two-channel polarization controller for Q-switching free mode-locked operation. The whole nonlinear microscope imaging system has the main advantages of the low price of the fs laser applied, fiber optics flexibility, a relatively small, light-weight scanning and detection head, and a very low risk of thermal or photochemical damage of the skin samples.

 

Minden érdeklődőt szívesen látunk!

Asbóth János
szfi-seminar AT wigner.mta.hu